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B12 pairs naturally with other foundational micronutrients in an endurance stack

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As the NCs are sensitive to detergents and can react with free thiols commonly present in the buffers used in sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), native PAGE was applied in these experiments

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A Perkin Elmer Gene Amp PCR System 9700 was used with the following cycling conditions: 94 C for 3 min, followed by two cycles of 95 C for 30 s, 60 C for 30 s and 72 C for 30 s followed immediately by the two same cycles with an auto-decrement temperature of 1 C until 51 C, and then 20 cycles of 95 C for 30 s, 50 C for 30 s, 50 C for 30 s and final extension at 72 C for 7 min