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2.2 RNA isolation and real-time polymerase chain reaction (PCR) analysis The total RNA was isolated from the heart apex tissue using Tri reagent (Sigma-Aldrich)

Quercetin protects human-derived liver cells against mercury-induced DNA-damage and alterations of the redox status

Although TNF at this dose exerted no cytotoxic effects, H 2 O 2 pretreatment resulted in marked cell death in both HepG2 cells and NCTC1469 cells in response to TNF challenge (Fig

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