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10.1523/jneurosci.19-10-03952.1999 125 WrightJ.YamamotoB.HardingJ

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The supernatant was discarded, and the pellet resuspended in cold PBS + 1% Triton X-100 plus protease and phosphatase inhibitor cocktails, transferred to an Eppendorf tube and allowed to rotate for 30 minutes at 4C